Hydroxamic Acid Glucosyltransferases from Maize Seedlings

Adenosine diphosphoglucose-starch glucosyltransferases from developing kernels of waxy maize.

TWO ADENOSINE DIPHOSPHOGLUCOSE: alpha-1,4-glucan alpha-4-glucosyl-transferases were extracted from kernels of waxy maize harvested 22 days after pollination and separated by gradient elution from a diethylaminoethyl-cellulose column. Both fractions could utilize amylopectin, amylose, glycogen, maltotriose and maltose as primers. The rate of glucose transfer from adenosine diphosphoglucose to ra...

Enhanced Activity of the Soluble Ribonucleic Acid Polymerase from 2,4-Dichlorophenoxyacetic Acid-treated Maize Seedlings.

Characterization of dual specificity protein kinase from maize seedlings.

A protein kinase of 57 kDa, able to phosphorylate tyrosine in synthetic substrates pol(Glu4,Tyr1) and a fragment of Src tyrosine kinase, was isolated and partly purified from maize seedlings (Zea mays). The protein kinase was able to phosphorylate exogenous proteins: enolase, caseins, histones and myelin basic protein. Amino acid analysis of phosphorylated casein and enolase, as well as of phos...

Hydroxamic acid as anticancer agent

Hydroxamic acids serve as a monsulfhydril bisubstrate analog inhibition of fernesyl protein transferase (FPT), the enzyme catalysing fernesylation of p21ras. Tumor necrosis factor (TFN) is specifically prevented by synthetic hydroxamic acid-based metalloproteinase inhibitors. Suberoyanilide hydroxamic acid (SAHA) have been identified as histone deacetylase inhibitors based on their ability to i...

Heterogeneous flavonoid glucosyltransferases in purple derivatives from a controlling element-suppressed bronze mutant in maize.

The Ds(Dissociation)-suppressed bronze mutant bz-m2(DI), which is extremely stable in the absence of the regulatory element Ac (Activator), becomes both somatically and germinally unstable when Ac is present. Instability in the germ line may result in the generation of stable (Ac-nonresponding), colored Bz' derivatives. The characterization of several properties of the Bz-controlled enzyme UDPg...